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Fig. 6 | Thrombosis Journal

Fig. 6

From: LINC00659 exacerbates endothelial progenitor cell dysfunction in deep vein thrombosis of the lower extremities by activating DNMT3A-mediated FGF1 promoter methylation

Fig. 6

Interference with FGF1 reversed the facilitative effect of LINC00659 inhibition on endothelial progenitor cell migration and angiogenesis. LINC00659 siRNA and FGF1 siRNA were transfected individually or together into EPCs. A Western blotting was applied to determine the levels of EIF4A3 in EPCs transfected with FGF1 siRNA or/and LINC00659 siRNA (n = 6). B The cell proliferation of EPCs transfected with FGF1 siRNA or/and LINC00659 siRNA was tested by CCK-8 assay (n = 6). C Transwell detection of the cell migration of EPCs transfected with FGF1 siRNA or/and LINC00659 siRNA (n = 6). D Tube formation assay was employed to check the effects of LINC00659 siRNA or/and FGF1 siRNA on EPC angiogenesis (n = 6). E-F Effect of LINC00659 siRNA or/and FGF1 siRNA on actin cytoskeleton structure in cultured EPCs (n = 4). Each experiment was repeated for 3 times. **P < 0.01.

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Thrombosis Journal

ISSN: 1477-9560

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