Fig. 3
From: Circular RNA ciRs-126 promotes hypoxia/reoxygenation cardiac injury possibly through miR-21
CiRs-126 overexpression decreased miR-21 expression in H/R cell model but not in non H/R treated cells. To explore the crosstalk between ciRs-126 and miR-21, ciRs-126 expression vector or miR-21 mimic was transfected into H/R-treated cardiomyocytes and non H/R treated cardiomyocytes. Overexpression of ciRs-126 and miR-21 was confirmed by RT-qPCR (A). The effects of ciRs-126 overexpression on miR-21 expression (B) and the effects of miR-21 overexpression on ciRs-126 (C) in both H/R-treated cardiomyocytes and non H/R treated cardiomyocytes were also analyzed by RT-qPCR. The cirRNA or miRNA expression in “C” of untreated cells was set as “1” and used to normalize expressions in other groups. Data of three biological replicates of cell transfection groups were expressed as mean±SD. *, p<0.05