Fig. 1
From: GDF15 affects venous thrombosis by promoting EndMT through smad2/p-smad2 pathway
DVT modeling process and thrombus morphology in mice. (A) Mouse DVT model, ligation under the mouse inferior vena cava left renal vein. Thrombus specimens of mice 1 week after modeling; (B) Masson staining and Sirius red staining of control group and VTE group. The scale was 200 μm (100×). (C) (D) Expression of GDF15 in serum and tissue and EndMT marker in tissue. Detection of serum concentration of GDF15 by Elisa. Immunohistochemistry and RT-qPCR showed that GDF15 was highly expressed in the vascular endothelium in the VTE group, and the scale x10 was 200 μm (100×), 50 μm (400×). (D) (E) Fluorescence staining and RT-qPCR detection of mouse blood vessel sections showed increased expression of EndMT marker SM22, immunofluorescence blue was DAPI, green was SM22, the scale was 50 μm (400×). * p < 0.05