Fig. 3

CAT calibration algorithm distorts TG curves when the fluorescence signal extends into the non-linear range of the calibrator. Thrombin peak height (TPH) and endogenous thrombin potential (ETP; measured as area under the curve) values were obtained from data in Fig. 1. TPH values from indicated software apps in (A) FVIII-DP supplemented with 1 IU/mL FVIII and (B) FVIII-DP with added buffer. ETP values from indicated software apps in (C) FVIII-DP supplemented with 1 IU/mL FVIII and (D) FVIII-DP with added buffer. Internal linear calibration is shown as black curves, CAT calibration is shown as blue curves (OR software), red curves (TS software), and green curves (SH software). CAT calibration correction did not return values at AMC concentrations > 115 µM. (E) TPH values from FVIII-DP supplemented with 1 IU/mL FVIII and (F) FVIII-DP with added buffer in indicated software apps, where p < 0.05 was deemed significantly different via a paired T-test (denoted as *) as compared to the Internal Linear group. Correlations of TPH from (G) TS software vs. OR Software, (H) SH software vs. OR Software, and (I) SH software vs. TS software in plasma with 0 IU/mL FVIII (gray squares) and with 1 IU/mL FVIIII (black squares). Assay conditions: 63 µL of FVIII-DP, 1 µL of FVIII (1 IU/mL), 16 µL of AMC at indicated concentrations, 20 µL of PPP trigger, and 20 µL of FluCa.