Table 1 Edge of failure set points for fluorescent artifacts on TG analysis. The significance the IFE and substrate consumption on TG as analyzed by three software packages: TS, OR, and SH

IFE

FVIII-DP spiked with [AMC]: 0, 47, 70, 102, 200 µM

FVIII = 1 IU/mL:

Corrected at [AMC] < 115 µM

FVIII = 0 IU/mL:

Corrected [AMC] < 75 µM

FVIII = 1 IU/mL:

Overestimated results at [AMC] > 170 µM

FVIII = 0 IU/mL:

Corrected [AMC] > 170 µM

FVIII = 1 IU/mL:

Successful at all AMC concentrations

FVIII = 0 IU/mL:

Successful at all AMC concentrations

FVIII = 1 IU/mL:

Successful at all spiked [AMC]

FVIII = 0 IU/mL:

Successful at all spiked [AMC]

Substrate Consumption*

Reduced ZGGR-AMC at range: 0-836 µM

n/a*†

n/a*

n/a*

n/a*

Reduced ratio of ZGGR-AFC supplemented to ZGGR-AMC

ZGGR-AFC: 0, 200, 400, 600, 700, 750, 800 µM

n/a*†

n/a*

n/a*

n/a*

Procoagulant Samples

ATIII-DP ± heparin with range of TF concentrations successfully induced substrate consumption

n/a†

Not corrected at any TF concentration

Successfully corrected at all TF concentrations

Corrected at all TF concentrations but lag time was modulated