Figure 1

(A) Upregulation of TF mRNA in HUVECs stimulated with bFGF (100 ng/ml) for 4 hr. The expressions of TF mRNA and of β-actin were determined by Northern blot analysis as described in Methods (n = 3). A representative autoradiograph is shown. (B) Effects of bFGF on concentrations of TF in HUVECs. Confluent cells were serum starved for 24 hr and then incubated with fresh serum free media containing bFGF (0 – 100 ng/ml) for 4 hr. Concentrations of TF in cell lysates were assayed by Western blotting as described in Methods (n= 6). The upper panel shows a representative blot from six separate experiments. Values are means ± SD of fold increase over control without bFGF. *P < 0.05 as compared to control. (C) Upregulation of TF mRNA in ECV cells stimulated with bFGF (0–100 ng/ml) for 4 hr. Expressions of TF mRNA and of β-actin were determined by Northern blot analysis as described in Methods (n = 3). A representative autoradiograph is shown. (D) Effects of bFGF on concentrations of TF in ECV cells. Confluent cells were serum starved for 24 hr and then incubated with fresh serum free media containing bFGF (0 – 100 ng/ml) for 4 hr. Concentrations of TF in cell lysates were assayed by Western blotting as described in Methods (n = 6). The upper panel shows a representative blot from six separate experiments. Values are means ± SD of fold increase over control without bFGF. *P < 0.05 as compared to control.