Figure 4
From: VacA, the vacuolating cytotoxin of Helicobacter pylori, binds to multimerin 1 on human platelets
Typical trace of GST-MMRN1-VacA association and dissociation. GST-MMRN1 was covalently coupled to CM5 chip using an Amine Coupling Kit. A control surface was reacted with amine coupling reagent in the absence of ligand. Several concentrations of VacA was perfused over the control surface and immobilized GST-MMRN1 surfaces, and the resonance changes were recorded and analysed with a BIAcore system (n=2).