Figure 6

Pxn-KD in platelets expedites thrombus formation in vivo. (A) Intravital imaging of thrombus formation by laser irradiation of mesenteric arterioles in mice with control or Pxn-KD platelets. Thrombus formation was increased in mice with Pxn-KD platelets following laser irradiation. Bar, 10 μm. The right panel shows the results of quantification of the thrombus area. (B) Percentage areas of thrombus within blood vessels after laser irradiation. Columns and error bars represent the mean ± s.e.m. (n = 40 vessels in five mice/group). (C) Tail bleeding times were assessed as described in the Methods. Columns and error bars represent the mean ± s.e.m. (n = four mice/group). Statistical significance was determined using Student’s t-test. *P < 0.05 vs. control.