Figure 7
Effect of specific blocking peptides designated P1 (binds to CD54) and P2 (binds to αMβ2) on sFn inhibition of monocyte/tumor cell adherence under flow conditions. (from left to right): sFn pre-treatment of monocytes and A375 cells (n = 25) significantly (P < 0.01 compared to untreated control; n = 30) inhibited monocyte adherence. Pretreatment of cells with P1 and P2 restored cell adherence to levels not significantly different to that of the untreated control (P < 0.05 to fibrin (n = 10); P > 0.05 to control). Pre-treatment of tumor cells with P1 and monocytes with sFn or tumor cells with sFn and monocytes with P2 inhibited adherence to a similar level to that of sFn treatment of both cells (P > 0.05 to fibrin; n = 5 in each case). Pretreatment of effector and tumor cells with fibrinogen (Fg), thrombin or GPRP did not significantly inhibit adherence (P > 0.05 compared to untreated cells; n = 5)