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Table 2 The comparison of methods used for PRP preparation in rabbits

From: Preparation of platelet-rich plasma as a tissue adhesive for experimental transplantation in rabbits

Study

Rabbit

Blood Samples

AC

Centrifugation

Hct

PRP

Platelets cells Count

EF

Activation type

Adhesive Test

 

T

F

M

S

ml

Type

1st

2nd

%

ml

Vein

PRP

%

  

Efeoglu et al [20]

23

13

10

Vein

11

EDTA

300 g/10'

5000 g/5'

NS

NS

555.000

3.134.500

465

not done

not done

Butterfield et al [22]

12

0

12

Ear artery

21

citrate

150 g/20'

400 g/10'

NS

1

468.000

2.061.000

340

CaCl + BT

not done

Hokugo et al [28]

15

NS

NS

NS

10

citrate

2400 rpm/10'

3600 rpm/10'

NS

0,8

200.000

1.200.000

500

Fib + BT

not done

Ohya et al [29]

18

18

0

NS

30

NS

203 g/5'

1050 g/5'

NS

3

227.000

864.000

281

CaCl + BT

not done

Jung et al [30]

16

NS

NS

NS

9

citrate

150 g/20'

500 g/20'

NS

0,5

NS

NS

NS

CaCl + BT

not done

Present study

40

18

22

Heart

10

citrate

72 g/15'

1006 g/5'

41

1

320.133

807.564

152

CaCl

done

  1. T: Total; F: Female; M: Male; Hct: Hematocrit; S: Source; AC: Anticoagulation; EDTA: Ethylenediaminetetraacetic acid; NS: Not Specified; CaCl: calcium chloride; Fib: Fibrinogen; BT: Bovine Thrombin; EF: Enrichment Factor
  2. Observation: centrifugation values by Hokugo et al were reported in centrifuge rotor speed (rpm). Not enough data was available to make the conversion to times gravidity (g).