Figure 1
Influence of lung and liver tissue homogenate on thrombin generation in plasma. Lung (light grey curve) or liver (dark grey curve) homogenates were added to human normal plasma with additional TF (5 pM) and PL (4 μM) (Panel A) or in the absence of additional TF and PL (Panel B). For comparison, thrombin generation in human normal plasma triggered with 5 pM TF and 4 μM PL is plotted (white curve) in panels A, B, C, and DPanel C: Addition of lung (light grey curve) or liver (dark grey curve) homogenate to protein C deficient plasma (Panel C) in the presence of additional TF/PL. Panel D: Addition of lung (light grey curve) or liver (dark grey curve) homogenates from TMpro/pro transgenic animals to platelet-poor human plasma. Panels E and F: overview of the lag time and ETP derived from thrombin generation curves for tissue homogenates added to platelet-poor human plasma in de absence of additional TF/PL (white bars) or in the presence of additional TF/PL (black bars), tissue homogenates added to protein C-deficient plasma (grey bars), and tissue homogenates from TMpro/pro transgenic animals to platelet-poor human plasma (hatched bars). Bars represent mean ± SD from 6 tissue homogenates.