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Fig. 2 | Thrombosis Journal

Fig. 2

From: Analysis of the potential of cancer cell lines to release tissue factor-containing microvesicles: correlation with tissue factor and PAR2 expression

Fig. 2

Semi-quantitative analysis of PAR2 mRNA and protein in cell lines. a Total RNA was isolated from each cell lines (5 × 105 cells) and 100 ng used to determine the relative amount of PAR2 mRNA by real-time RT-PCR using β-actin mRNA as reference. The ratios of PAR2 mRNA were determined with respect to that expressed by MIA-PaCa-2 cells. (n = 3) b The relative amount of cellular PAR2 was assessed in lysed non-activated cells (5 × 105) analysed by western blot and probed with an antibody against human PAR2 (SAM11) diluted 1:1000 (v/v) in TBST, developed with a goat anti-mouse HRP-conjugated antibody and visualised using the TMB substrate. The level of GAPDH was measured using an anti-human GAPDH antibody (V-18) followed by a donkey anti-goat alkaline phosphatase-conjugated antibody and visualised as above. (n = 3)

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Thrombosis Journal

ISSN: 1477-9560

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