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Fig. 4 | Thrombosis Journal

Fig. 4

From: Complement C5a induces the generation of neutrophil extracellular traps by inhibiting mitochondrial STAT3 to promote the development of arterial thrombosis

Fig. 4

Interactions among C5a, mitochondrial STAT3 and NETs. A, B Western blot analysis was performed to test the expression levels of mitochondrial STAT3 and p-STAT3 (Ser727) in neutrophil-like cells cocultured with C5a, compared with the control. VDAC, a marker of mitochondria, was used as a loading control for mitochondria (n = 5 each). C NET release in response to buffer or AG490 was measured using a plate reader assay (n = 3 each). D Representative images of immunofluorescence staining for DNA (SYTOX green), CitH3 (red) and Ly6g (blue) in vitro after stimulation with AG490 showing the presence of NETs. Scale bar = 100 µm. E Quantification of NET formation capacity shown as the percentage of NET release in vitro after stimulation with buffer or AG490, as assessed by immunofluorescence staining. (n = 3 each). Data are presented as mean ± SD

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Thrombosis Journal

ISSN: 1477-9560

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