Table 1 Primer and probe sequences for the detection of PAI-1 and FVL polymorphisms
From: Clinical impact of the PAI-1 4G/5G polymorphism in Chinese patients with venous thromboembolism
Targets | Sequences |
|---|---|
PAI-1 4G/5G | |
 Forward primer | 5'-GCCAGACAAGGTTGTTGACACA-3' |
 Reverse primer | 5'-GAGGACTCTTGGTCTTTCCCTCAT-3' |
 5G allele probe | 5'-VIC-CTGACTCCCCCACGTG-MGB-NFQ-3' |
 4G allele probe | 5'-FAM-CTGACTCCCCACGTGT-MGB-NFQ-3' |
F5 G1691A (FVL) | |
 Forward primer | 5'-GACCATACTACAGTGACGTGGACATC-3' |
 Reverse primer | 5'-CCCATTATTTAGCCAGGAGACCTAAC-3' |
 G allele probe | 5'-VIC-TATTCCTCGCCTGTCCA-MGB-NFQ-3' |
 A allele probe | 5'-FAM-ACCTGTATTCCTTGCCTG-MGB-NFQ-3' |
β-Actin | |
 Forward primer | 5'-TGAAGATCCTCACCGAGCG-3' |
 Reverse primer | 5'-GGCAGCTCGTAGCTCTTCTC-3' |
 Probe | 5'-ROX-ACCACCACGGCCGAGCGG-BHQ-3' |