Table 2 Methodological comparison of ECLT and GFC
From: Laboratory evaluation of a new integrative assay to phenotype plasma fibrinolytic system
Euglobulin clot lysis time | Global fibrinolysis capacity | |
|---|---|---|
Preanalytical | Binding, with sample collection, centrifugation and processing on melting ice to keep it at 4 °C | Sample collection, centrifugation and processing at room temperature |
Measurement | Plasma sample artificially depleted in fibrinolysis inhibitors to mainly keep the euglobulin fraction (mainly fibrinogen, plasminogen and plasminogen activators (t-PA and u-PA)) | ‘Native’ plasma with fibrinolysis activators and inhibitors |
Clot formation | Triggered by addition of CaCl2 → Endogenous thrombin → Endogenous fibrinogen | Triggered by addition of CaCl2 and exogenous human thrombin in large amount → Exogenous human thrombin → Endogenous fibrinogen |
Clot lysis | Natural | Induced by the addition of micronized silica and recombinant t-PA (100 ng/mL) |
Locally established reference range | 131 – 523 min | 29.3 – 49.5 min |