Fig. 2

Silencing of Crnde attenuates vascular inflammatory injury, thereby curtailing DVT. A, RT-qPCR detection of Crnde expression in the inferior vena cava tissues of the sh-Crnde-treated DVT mice. B, Representative image of thrombosis in inferior vena cava of the DVT mice in response to Crnde knockdown. C & D, Measurement results of length (C) and weight (D) of thrombus in the DVT mice in response to Crnde knockdown. E, Representative micrographs of thrombus (F4/80) and myeloperoxidase staining for the DVT mice in response to Crnde knockdown. F, Quantification of F4/80 positive cells per unit area in the inferior vena cava tissues of the DVT mice in response to Crnde knockdown. G, Quantification of myeloperoxidase staining-positive cells per unit area in the inferior vena cava tissues of the DVT mice in response to Crnde knockdown. H-J, The levels of inflammatory factors IL-1β (H), IL-1, IL-6 (I) and IL-8 (J) in the femoral venous blood of the DVT mice in response to Crnde knockdown. K, The vascular inflammatory injury and platelet bundles in the DVT mice in response to Crnde knockdown. Red triangles indicate clustered red blood cells, and yellow arrows indicate the blood vessel wall. ** p < 0.01, *** p < 0.001 vs. the sh-NC group. n = 6