Fig. 4

Overexpression of miR-181a-5p attenuates vascular inflammatory injury, thereby curtailing DVT. A, RT-qPCR detection of miR-181a-5p expression in the inferior vena cava tissues of the sham-operated mice and DVT mice. B, RT-qPCR detection of miR-181a-5p expression in the inferior vena cava tissues of the miR-181a-5p agomir-treated DVT mice. C, Representative image of thrombosis in inferior vena cava of the miR-181a-5p agomir-treated DVT mice. D & E, Measurement results of length (D) and weight (E) of thrombus in the miR-181a-5p agomir-treated DVT mice. F, Representative micrographs of thrombus (F4/80) and myeloperoxidase staining for the miR-181a-5p agomir-treated DVT mice. G, Quantification of F4/80 positive cells per unit area in the inferior vena cava of the miR-181a-5p agomir-treated DVT mice. H, Quantification of myeloperoxidase staining-positive cells per unit area in the inferior vena cava of the miR-181a-5p agomir-treated DVT mice. I-K, The levels of inflammatory factors IL-1β (I), IL-1, IL-6 (J) and IL-8 (K) in the femoral venous blood of the miR-181a-5p agomir-treated DVT mice. L, The vascular inflammatory injury and platelet bundles in the miR-181a-5p agomir-treated DVT mice. Red triangles indicate clustered red blood cells, and yellow arrows indicate the blood vessel wall. ** p < 0.01, *** p < 0.001 vs. the sham group or agomir NC group. n = 6