Fig. 6

Overexpression of Pcyox1l reverses the inhibitory effect of Crnde silencing on DVT in mice. A, The expression of Crnde, miR-181a-5p and Pcyox1l in DVT mice treated with sh-Crnde alone or combined with oe-Pcyox1l determined by RT-qPCR. B, Western blot for the Pcyox1l protein expression in DVT mice treated with sh-Crnde alone or combined with oe-Pcyox1l. C & D, Images (C) and quantitation (D) of Pcyox1l protein expression in the inferior vena cava tissue of the DVT mice treated with sh-Crnde alone or combined with oe-Pcyox1l determined by immunohistochemistry. E, Representative image of thrombosis in inferior vena cava of the DVT mice in response to Crnde knockdown alone or combined with Pcyox1l overexpression. F & G, Measurement results of thrombus length (F) and thrombus weight (G) of the DVT mice in response to Crnde knockdown alone or combined with Pcyox1l overexpression. H-J, The levels of inflammatory factors IL-1β (H), IL-6 (I) and IL-1 and IL-8 (J) in the femoral venous blood of the DVT mice in response to Crnde knockdown alone or combined with Pcyox1l overexpression measured by ELISA. K, HE staining showing vascular inflammatory injury and platelet bundles in the DVT mice in response to Crnde knockdown alone or combined with Pcyox1l overexpression. Red triangles indicate the clustered red blood cells, and yellow arrows indicate the blood vessel wall. *** p < 0.001 vs. the sh-NC + oe-NC group. ### p < 0.001 vs. the sh-Crnde + oe-NC group. n = 6