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Fig. 1 | Thrombosis Journal

Fig. 1

From: Hemostatic potential of recombinant von Willebrand factor and standard or pegylated extended half-life recombinant factor VIII on thrombus formation under high shear flow

Fig. 1

The impact of rVWF and rFVIII on thrombus formation in type 1 VWD whole blood under high shear flow. (Panel A) rVWF (Vonvendi®; 1.6 IU/mL) and/or rFVIII (Advate®; 0–1.0 IU/mL), was mixed with whole blood from patients with type 1 VWD, and perfused in the collagen coated perfusion chambers under high shear (2,500 s− 1). pd-VWF/FVIII (Confact®; VWF:FVIII ratio; 1.6 IU/mL:1.0 IU/mL) and PBS were used as positive or negative controls, respectively. Thrombi were fixed after 4 min perfusion. Platelets were stained with phalloidin-Alexa 488 (6 µg/mL) and were observed using confocal laser scanning microscopy. Representative images are illustrated, and statistical analyses corresponding to the images are shown. The scale bar is set at 30 μm. (Panel B); Values and error bars represent the mean(± SD) of surface coverage and thrombus height in 15 defined areas (each 133 × 100 μm) randomly selected in 3 separate flow experiments. *p < 0.05, **p < 0.01, ***p < 0.001

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Thrombosis Journal

ISSN: 1477-9560

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