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Fig. 2 | Thrombosis Journal

Fig. 2

From: Hemostatic potential of recombinant von Willebrand factor and standard or pegylated extended half-life recombinant factor VIII on thrombus formation under high shear flow

Fig. 2

The influence of pre-incubation of rVWF with rFVIII on thrombus formation in type 1 VWD whole blood under high shear flow. (Panel A) rVWF (1.6 IU/mL) and/or rFVIII (1.0 IU/mL), was incubated with type 1 VWD whole blood for the indicated times (0, 15, and 30 min) and perfused in the collagen coated perfusion chamber under high shear (2,500 s− 1). PBS was used as a negative control. Thrombi were fixed after 4 min perfusion. Platelets were stained with phalloidin-Alexa 488 (6 µg/mL) and were observed using confocal laser scanning microscopy. Representative images at the indicated pre-incubation times are illustrated, and statistical analyses corresponding to the images are shown. The scale bar is set at 30 μm. (Panel B); Values and error bars represent the mean(± SD) of surface coverage and thrombus height in 15 defined areas (each 133 × 100 μm) randomly selected in 3 separate flow experiments. *p < 0.05, **p < 0.01, ***p < 0.001

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Thrombosis Journal

ISSN: 1477-9560

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