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Fig. 3 | Thrombosis Journal

Fig. 3

From: Hemostatic potential of recombinant von Willebrand factor and standard or pegylated extended half-life recombinant factor VIII on thrombus formation under high shear flow

Fig. 3

The association between rVWF and peg-EHL-rFVIII in formed thrombi after perfusion at in type 1 VWD whole blood under high shear flow. (Panel A) rVWF (1.6 IU/mL), rFVIII (1.0 IU/mL) and/or peg-EHL-rFVIII (1.0 U/mL) were added to whole blood from patients with type 1 VWD, and perfused in the collagen coated chambers under high shear (2,500 s− 1). pd-VWF/FVIII (1.6 IU/mL/1.0 IU/mL) and PBS were used as positive or negative controls, respectively. Thrombi were fixed after 4 min perfusion. Platelets were stained with phalloidin-Alexa 488 (6 µg/mL) and were observed using confocal laser scanning microscopy. Representative images of PBS, peg-EHL-rFVIII, rVWF/peg-EHL-rFVIII are illustrated, and statistical analyses corresponding to the images are shown. The scale bar is set at 30 μm. (Panel B) Values and error bars represent the mean(± SD) of surface coverage and thrombus height in 15 defined areas (each 133 × 100 μm) randomly selected in 3 separate flow experiments. *p < 0.05, **p < 0.01, ***p < 0.001, EHL-rFVIII; peg-EHL-rFVIII

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Thrombosis Journal

ISSN: 1477-9560

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