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Fig. 5 | Thrombosis Journal

Fig. 5

From: Hemostatic potential of recombinant von Willebrand factor and standard or pegylated extended half-life recombinant factor VIII on thrombus formation under high shear flow

Fig. 5

Localization of rVWF, rFVIII, and peg-EHL-rFVIII in formed thrombi in type 1 VWD whole blood at high shear flow. rVWF (1.6 IU/mL), rFVIII (1.0 IU/mL) and/or peg-EHL-rFVIII (1.0 IU/mL) were added to type 1 VWD whole blood from perfused in collagen coated perfusion chamber under high shear (2,500 s− 1). pd-VWF/FVIII and PBS (VWF:FVIII = 1.6 IU/mL:1.0 IU/mL) were used as positive or negative controls, respectively. Thrombi were fixed after 4 min perfusion, and stained with phalloidin-Alexa 488 (green), anti-VWF antibody-Alexa 567 (red pseudo-color), and anti-FVIII antibody-Alexa 647 (blue pseudo-color). Representative images are illustrated. Combined color images are defined as merged. The scale bar is set at 30 μm. EHL-rFVIII; peg-EHL-rFVIII

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Thrombosis Journal

ISSN: 1477-9560

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