Fig. 3

Qualitative co-localisation of TF and MAGI1 in resting and activated MDA-MB-231 cells. MDA-MB-231 cells (10⁴) were seeded out into 35 mm-glass based μ-dishes and sets were activated by incubation with PAR2-AP (20 μM). The cells were fixed and probed for TF using an FITC-conjugated anti-TF antibody (HTF1-FITC). The patterns were compared to MAGI1 probed with a rabbit anti-MAGI1 antibody (H-70) and developed a goat anti-rabbit IgG-AlexaFluor 594 antibody. The cells were also stained with DAPI (2 μg/ml) and examined by fluorescence microscopy. Co-localisation coefficient values were then determiend using the ImageJ program. The values were calculated as the average from 10 captured images, and the data show the values for 5 separate experiments