Fig. 1

Representative agarose gels of RFLP PCR. (A) BFG-455G > A mutation: HaeIII digestion of PCR product (446 bp) yields 348 and 98 bp for the wild type allele or 446 bp for the mutant allele. Lane L: 50 bp DNA ladder; lanes 2, 4 and 5 are normal; lanes 1, 3 and 6 are heterozygote; and lane 7 is mutant homozygote. (B) FII G20210A mutation: HindIIII digestion of PCR products yields 264 bp for the wild type allele and 232 and 32 bp for the mutant allele. Lane L: 50 bp DNA ladder; lanes 1–5 are normal; lanes 6 and 7 are heterozygotes. (C) FVL mutation: HindIII digestion of PCR products yield 241 bp for the wild type allele and 209 and 32 bp for the mutant allele. Lane L: 50 bp DNA ladder; lanes 1–5 and 7 are normal; lane 6 is heterozygote; and lane 8 is mutant homozygote. (D) MTHFR C677T mutation: HinfI digestion of PCR products yields 198 bp for the wild type allele and 175 and 23 bp for the mutant allele. Lane L: 50 bp DNA ladder; lanes 1, 2, 4 and 5 are normal; lane 7 is heterozygotes; and lanes 3 and 6 are homozygote mutants. (E) MTHFR A1298C mutation: MboII digestion of PCR products yields 56, 31, 30, 28 and 18 bp for the wild type allele and 84, 31, 30 and 18 bp for the mutant allele. Lane L: 50 bp DNA ladder; lane 3 is normal; lanes 1, 4 and 6 are heterozygotes; and lanes 2 and 5 are homozygote mutants