- Open Access
Relationship between ABO blood group and von Willebrand factor levels: from biology to clinical implications
© Franchini et al; licensee BioMed Central Ltd. 2007
Received: 06 August 2007
Accepted: 25 September 2007
Published: 25 September 2007
Although a number of studies have demonstrated the influence of ABO blood group on plasma levels of von Willebrand factor (VWF), the nature of this association and its clinical importance is still largely unknown.
In this review, the most recent advances in our understanding of the mechanisms by which ABO blood group determines plasma VWF levels and their clinical impact will be discussed.
VWF has two major functions in hemostasis. First, it is essential for platelet-subendothelium adhesion and platelet-to-platelet interactions as well as platelet aggregation in vessels in which rapid blood flow results in elevated shear stress. Second, VWF is the specific carrier of factor VIII (FVIII) in plasma and protects it from proteolytic degradation, prolonging its half-life in circulation and efficiently localizing it at the site of vascular injury .
While a deficiency of VWF is responsible for a hemorrhagic diathesis (von Willebrand disease, VWD) , there are increasing evidences that elevated VWF levels represent an important thrombotic risk factor [6, 7].
Besides the VWF gene (12p12), it is well established that other gene loci exert major quantitative effects on VWF plasma levels. The most important of these loci has been shown to be the ABO blood group locus on chromosome 9q34 [8, 9].
The antigens of the ABO system (A, B, and H determinants, respectively) consist of complex carbohydrate molecules. The A and B alleles encode slightly different glycosyltransferases that add N-acetylgalactosamine and D-galactose, respectively, to a common precursor side chain, the H determinant, converting it into A- or B-antigens. The O alleles do not encode a functional enzyme and consequently OO carriers, who lack such transferase enzymes, continue to express the basic, unmodified, H- antigen with a fucose moiety attached to precursor oligosaccharide chains.
The nature of the interaction between ABO blood group antigens and plasma VWF levels along with its clinical implication will be discussed in this review.
Studies on the influence of ABO blood groups on VWF levels
Several studies have documented the influence of ABO blood groups on plasma VWF levels [11–13]. In a large twin study, Orstavik and colleagues  found that 66 percent of the total variation in plasma VWF levels was genetically determined and that 30 percent of this genetic component was explained by ABO blood group. Other studies have consistently reported that group O subjects have lower plasma VWF levels than non-O individuals. Indeed, in a large study of 1117 healthy individuals conducted by Gill and colleagues , plasma VWF levels were lowest in group O (mean von Willebrand factor antigen [VWF:Ag] 75 IU/dL) and highest in group AB subjects (mean VWF:Ag 123 IU/dL). From the same study also emerged that the influence of ABO blood groups on plasma VWF levels can make difficult the diagnosis of type 1 VWD since the normal range for VWF:Ag in group O individuals extends below 50 IU/dL (mean ± 2SD: 36–157 IU/dL), which is commonly accepted as the lower normal limit. In fact the authors, investigating the distribution of ABO blood groups in 114 patients with type 1 VWD, found a predominance of blood group O with a frequency significantly different from the general population (77% group O, 18% group A, 4% group B in type 1 VWD patients versus 45% group O, 45% group A, 7% group B in healthy individuals, P < 0.001) . However, the primary importance of the bleeding history in the clinical decision to diagnose and treat type 1 VWD was stressed by Nitu-Whalley and colleagues in a retrospective study on the re-classification of 246 previously diagnosed type 1 VWD patients .
In addition to the above mentioned VWF antigenic variability, Moeller and colleagues  described variations in VWF activity (von Willebrand factor ristocetin cofactor, VWF:RCo) in persons with different ABO groups with individuals of group O that exhibited VWF:RCo levels significantly lower than those in the non-O group.
Miller and colleagues  studied the effect of ABO blood type and race on plasma VWF levels and found that Caucasians had significantly lower levels than African-Americans. Interestingly, ABO and race showed independently effects accounting for 19 and 7 percent of the total variance in VWF:Ag levels, respectively.
Besides the studies on ABO phenotype, other investigators have examined the relationship between ABO genotype and plasma VWF levels [19–21]. According with previous studies, lowest plasma VWF levels were present in genotype OO individuals. However, all groups also found that individuals heterozygous for the O allele (genotypes AO, BO) had significantly lower plasma VWF levels then those not carrying an O allele (genotypes AA, AB, BB).
Finally, other investigators studied the influence of Secretor blood group, characterized by a fucose as terminal sugar, on plasma VWF levels and found significantly higher VWF levels in individuals homozygous for the Se allele (genotype SeSe) compared to those heterozygous (genotype Sese) .
The nature of the association
Von Willebrand factor is one of the few non-erythrocytic proteins that express ABO antigens. ABH oligosaccaride structures have been identified on the N-linked oligosaccharide chains of VWF located in the A1 domain, which contains the binding site for platelet glycoprotein Ib (GPIb) I . The importance of VWF glycosilation in mediating the effect of ABO blood group on VWF levels is well demonstrated by the observation that aberrant endothelial expression of a glycosyltranferase (N-acetylgalactosaminyltranferase) results in altered VWF glycosilation and heightened clearance in mice . O'Donnell and colleagues found a direct relationship between ABO genotype, A transferase expression and the amount of A antigen expressed on circulating VWF . Similarly, Morelli and colleagues  demonstrated an allele specific, dosage dependent effect of the ABO alleles on VWF and FVIII levels and on the degree to which VWF was loaded with A and B antigens. Furthermore, A and B antigens expressed on VWF explained about 18 percent of the variation in plasma VFW levels.
The mechanism by which ABH determinants on plasma VWF influence plasma VWF levels is still unknown and several hypotheses have been made during the last years . While the in vitro removal of A and B antigens from purified plasma VWF decreases VWF activity but not its antigenic level or binding to collagen , the detection of lower levels of all three in type O individuals suggests that ABO antigens in vivo influence the rate of synthesis or proteolysis/clearance of the whole VWF molecule rather than its function or multimeric structure .
The hypothesis that ABO blood group influences the rate of biosynthesis/secretion of VWF in endothelial cells is difficult to study, as glycan structures of VWF synthesized in vitro differ significantly from those of plasma VWF . Furthermore, in vivo data do not support this theory as the total increase in VWF after infusion of desmopressin was not significantly different between group O and non-O individuals .
However, a more probable explanation for reduced VWF levels in blood group O individuals involves a decreased survival.
A central role for VWF glycan in determining the rate of hepatic clearance has been demonstrated in both animal models and humans. Changes in the glycosylation pattern of VWF in a mouse strain with altered expression of an N-acetylgalactosaminyltransferase gene resulted in low VWF levels, possibly because of an accelerated clearance . Another direct link between VWF glycosylation and clearance comes from the study by Ellies and colleagues  on mice with absence of the enzyme ST3Gal-IV, which mediates the attachment of sialyl groups to terminal galactose residues. In ST3Gal-IV-deficient mice, the half-life of endogenous VWF was reduced two-fold. Similar results were observed by Sodetz and colleagues in rabbits . A similar association between reduced ST3Gal-IV-mediated sialylation and reduced plasma VWF levels was also found in humans . These data indicate that sialylation is important to prevent premature clearance via receptors that recognize non-sialylated terminal galactose residues, like the hepatic asialoglycoprotein receptor. Nossent and colleagues  used a mathematical approach and estimated that half-life of endogenous VWF is two hours longer than in the non-O population. Additional evidence that blood group O determinants on VWF are associated with increased clearance is provided by observations that infused FVIII (either plasma-derived or recombinant) disappears more rapidly in blood group O than in non-group O hemophilia A patients . As regards the mechanism by which ABH structures on VWF may influence clearance rates, O'Donnell et al. originally proposed that clearance of VWF is mediated via the H- antigen by hepatic receptors specific for fucose exposing glycoproteins . However, the subsequent finding that subjects with the Bombay phenotype, who totally lack H-antigen on VWF and are non-secretors (no H substance in plasma), have even lower VWF levels than blood group OO carriers seems to be in conflict with this model .
Recent studies have suggested that ABO blood group determinants may be important in influencing the susceptibility of plasma VWF to proteolysis by ADAMTS13 (a disintegrin and metalloproteinase with thrombospondin type-1 repeats-13) metalloprotease . Bowen  purified VWF from individuals of different ABO blood groups and then incubated with human plasma-derived ADAMTS13. Mulimeric analysis and collagen-binding assays both demonstrated that proteolysis was significantly faster for group O VWF compared to non-O VWF. Thus, while carbohydrate structure has previously been shown to protect VWF from proteolytic degradation in plasma , the recent literature data show that a reduction in the number of sugars on the oligosaccharide chains of VWF is associated with an increased susceptibility to cleavage by ADAMTS13 . Indeed, Bombay phenotype was found to be associated with an increased susceptibility to ADAMTS13 proteolysis . The pathogenic mechanism by which ABO blood group influences the susceptibility to cleavage by ADAMTS13 is still unknown but two N-linked potential glycosilation sites (asparagines 1515 and 1574) are located in close proximity to the ADAMTS13 cleavage site (Tyr1605-Met1606 bond within the A2 domain). Thus, the oligosaccharide chain composition may be involved in stabilizing the conformation of this VWF region, such that the removal of terminal sugar allows the A2 domain to adopt a conformation more permissive for ADAMTS13 cleavage.
A number of studies have demonstrated a relationship between ABO blood group and hemostasis. Indeed, a higher rate of bleeding complications has been described in patients belonging to group O [37, 38] and blood group O individuals are consistently overrepresented in patients with inherited bleeding disorders . By contrast, an association between non-O blood groups and the risk of arterial thromboembolic disease, including ischemic heart disease and peripheral vascular disease, has been recognised by several studies [39–42]. Results from the Hoorn study  indicated that blood group non-O was associated with a twofold increased cardiovascular mortality compared with blood group O (RR 2.08 [95% CI 0.85–5.07]). The increased risk of venous thromboembolism in non-O individuals has also been reported [44–47]. Interestingly, Morelli and colleagues  found that carriers of non-O alleles had a twofold increased risk of a first deep vein thrombosis and that the non-OO genotypes strongly influenced the risk of thrombosis in FV Leiden carriers. On the other hand, both arterial and venous thrombotic risks have been related to VWF levels [49–51]. However, only few studies have examined the effect of VWF levels on the association between ABO blood type and thrombosis.
To elucidate the role of the ABO blood group, VWF and FVIII in the process of deep vein thrombosis, a population-based patient-control study on 301 consecutive patients and 301 controls was performed by Koster and colleagues . In univariate analysis the authors found that the risk of thrombosis increased with increasing VWF and FVIII levels and was higher in non-O subjects than in those of group O. However, in multivariate analysis only FVIII remained as a risk factor, suggesting a close relationship between blood group and VWF concentration.
Tirado and colleagues  analyzed 250 patients with venous thrombosis and 250 unrelated controls and found higher VWF levels in non-O group, which was more frequent in patients. However, the risk attributed to VWF was strongly dependent on blood groups as it disappeared after adjusting for the ABO groups. Similar results were found by Ohira and colleagues  in the Longitudinal Investigation of Thromboembolism Etiology (LITE) study.
On the whole, the results of these studies indicate that, compared with type O, non-O individuals could have an increased thrombotic risk via having higher VWF-FVIII levels. However, not all studies agree with these findings as some authors found that the association between VWF and the risk of cardiovascular mortality was independent of blood group [40, 43].
A number of studies demonstrates that ABO blood group influences plasma levels of VWF. Recent investigations seem to indicate an altered susceptibility to cleavage by ADAMTS13 as an important mechanism by which ABO group can affect the rate of VWF catabolism. However, the elucidation of the physiology of glycan structures of VWF will help to understand the mechanisms by which blood group contribute to the thrombotic risk.
- Ruggeri ZM, Zimmerman TS: The complex multimeric composition of factor VIII/vWF. Blood 1981, 57: 1140-1143.PubMedGoogle Scholar
- Sadler JE: von Willebrand factor. Ann Rev Biochem 1998, 67: 395-424. 10.1146/annurev.biochem.67.1.395View ArticlePubMedGoogle Scholar
- Ruggeri ZM: Structure of von Willebrand factor and its function in platelet adhesion and thrombus formation. Best Pract ResClinical Haematol 2001, 14: 257-259. 10.1053/beha.2001.0133View ArticleGoogle Scholar
- Vlot AJ, Koppelman SJ, Bouma BN, Sixma JJ: Factor VIII and von Willebrand Factor. Thromb Haemost 1998, 79: 456-465.PubMedGoogle Scholar
- Ruggeri ZM, Zimmerman TS: von Willebrand factor and von Willebrand disease. Blood 1987, 70: 895-904.PubMedGoogle Scholar
- Whincup PH, Danesh J, Walker M, Lennon L, Thomson A, Appleby P, Rumley A, Lowe GD: von Willebrand factor and coronary heart disease: prospective study and meta-analysis. Eur Heart J 2002, 23: 1764-1770. 10.1053/euhj.2001.3237View ArticlePubMedGoogle Scholar
- Franchini M, Lippi G: Von Willebrand factor and thrombosis. Ann Hematol 2006, 85: 415-423. 10.1007/s00277-006-0085-5View ArticlePubMedGoogle Scholar
- O'Donnell JS, Lasffan MA: The relationship between ABO histo-blood group, factor VIII and von Willebrand factor. Transfus Med 2001, 11: 343-351. 10.1046/j.1365-3148.2001.00315.xView ArticlePubMedGoogle Scholar
- Jenkins PV, O'Donnell JS: ABO blood group determines plasma von Willebrand factor levels: a biologic function after all? Transfusion 2006, 46: 1836-1844. 10.1111/j.1537-2995.2006.00975.xView ArticlePubMedGoogle Scholar
- Lowe J: The blood group-specific human glycosyltransferases. Baillieres Clin Haematol 1993, 6: 465-490. 10.1016/S0950-3536(05)80155-6View ArticlePubMedGoogle Scholar
- Gastineau DA, Moore SB: How important are ABO-related variations in coagulation factor levels? Transfusion 2001, 41: 4-5. 10.1046/j.1537-2995.2001.41010004.xView ArticlePubMedGoogle Scholar
- McCallum CJ, Peake IR, Newcombe RG, Bloom AL: Factor VIII levels and blood group antigens. Thromb Haemost 1983, 50: 757.PubMedGoogle Scholar
- Mohanty D, Ghosh K, Marwaha N, Kaur S, Chauhan AP, Das KC: Major blood group antigens – a determinant of factor VIII levels in blood? Thromb Haemost 1984, 51: 414.PubMedGoogle Scholar
- Orstavik KH, Magnus P, Reisner H, Berg K, Graham JB, Nance W: Factor VIII and factor IX in a twin population: evidence for a major effect of ABO locus on factor VIII level. Am J Hum Genet 1985, 37: 89-101.PubMed CentralPubMedGoogle Scholar
- Gill JC, Endres-Brooks J, Bauer PJ, Marks WJ Jr, Montgomery RR: The effect of ABO blood group on the diagnosis of von Willebrand disease. Blood 1987, 69: 1691-1695.PubMedGoogle Scholar
- Nitu-Whalley IC, Lee CA, Griffioen A, Jenkins PV, Pasi KJ: Type 1 von Willebrand disease – a clinical retrospective study of the diagnosis, the influence of the ABO blood group and the role of the bleeding history. Br J Haematol 2000, 108: 259-264. 10.1046/j.1365-2141.2000.01830.xView ArticlePubMedGoogle Scholar
- Moeller A, Weippert-Kretschmer M, Prinz H, Kretschmer V: Influence of ABO blood groups on primary hemostasis. Transfusion 2001, 41: 56-60. 10.1046/j.1537-2995.2001.41010056.xView ArticlePubMedGoogle Scholar
- Miller CH, Haff E, Platt SJ, Rawlins P, Drews CD, Dilley AB, Evatt B: Measurement of von Willebrand factor activity: relative effects of ABO blood type and race. J Thromb Haemost 2003, 1: 2191-2197. 10.1046/j.1538-7836.2003.00367.xView ArticlePubMedGoogle Scholar
- Shima M, Fujimura Y, Nishiyama T, Tsujiuchi T, Narita N, Matsui T, Titani K, Katayama M, Yamamoto F, Yoshioka A: ABO blood group genotype and plasma von Willebrand factor in normal individuals. Vox Sang 1995, 68: 236-240.View ArticlePubMedGoogle Scholar
- Souto JC, Almasy L, Muniz-Diaz E, Soria JM, Borrell M, Bayen L, Mateo J, Madoz P, Stone W, Blangero J, Fontcuberta J: Functional effects of the ABO locus polymorphism on plasma levels of von Willebrand factor, factor VIII, and activated partial thromboplastin time. Arterioscler Thromb Vasc Biol 2000, 20: 2024-2028.View ArticlePubMedGoogle Scholar
- Sousa CM, Anicchino-Bizzacchi JM, Locatelli MF, Catsro V, Barjas-Castro L: The relationship between ABO groups and subgroups, factor VIII and von Willebrand factor. Haematologica 2007, 92: 236-239.View ArticlePubMedGoogle Scholar
- O'Donnell J, Boulton FE, Manning RA, Laffan MA: Genotype at the secretor blood group locus is a determinant of plasma von Willebrand factor level. Br J Haematol 2002, 116: 350-356. 10.1046/j.1365-2141.2002.03270.xView ArticlePubMedGoogle Scholar
- Matsui T, Titani K, Mizuochi T: Structures of the asparagine-linked oligosaccharide chains of human von Willebrand factor. Occurrence of blood group A, B, and H(O) structures. J Biol Chem 1992, 267: 8723-8731.PubMedGoogle Scholar
- Mohlke KL, Purkayastha AA, Westrick RJ, Smith PL, Petryniak B, Lowe JB, Ginsburg D: Mvwf, a dominant modifier of murine von Willebrand factor, results from altered lineage-specific expression of a glycosyltransferase. Cell 1999, 96: 111-120. 10.1016/S0092-8674(00)80964-2View ArticlePubMedGoogle Scholar
- O'Donnell J, Boulton FE, Manning RA, Laffan MA: Amount of H antigen expressed on circulating von Willebrand factor is modified by ABO blood group genotype and is a major determinant of plasma von Willebrand factor antigen levels. Arterioscler Thromb Vasc Biol 2002, 22: 335-341. 10.1161/hq0202.103997View ArticlePubMedGoogle Scholar
- Morelli VM, de Visser MC, van Tilburg NH, Vos HL, Eikenboom JC, Rosendaal FR, Bertina RM: ABO blood group genotypes, plasma von Willebrand factor levels and loading of von Willebrand factor with A and B antigens. Thromb Haemost 2007, 97: 534-541.PubMedGoogle Scholar
- Sarode R, Goldstein J, Sussman II, Nagel RL, Tsai HM: Role of A and B blood group antigens in the expression of adhesive activity of von Willebrand factor. Br J Haematol 2000, 109: 857-64. 10.1046/j.1365-2141.2000.02113.xView ArticlePubMedGoogle Scholar
- Lenting PJ, van Schooten CJM, Denis CV: Clearance mechanisms of von Willebrand factor and factor VIII. J Thromb Haemost 2007, 5: 1353-1360. 10.1111/j.1538-7836.2007.02572.xView ArticlePubMedGoogle Scholar
- Brown SA, Eldridge A, Collins PW, Bowen DJ: Increased clearance of von Willebrand factor antigen post-DDAVP in Type 1 von Willebrand disease: is it a potential pathogenic process? J Thromb Haemost 2003, 1: 1714-1717. 10.1046/j.1538-7836.2003.00359.xView ArticlePubMedGoogle Scholar
- Ellies LG, Ditto D, Levy GG, Wahrenbrock M, Ginsburg D, Varki A, Le DT, Marth JD: Sialyltransferase ST3Gal-IV operates as a dominant modifier of hemostasis by concealing asialoglycoprotein receptor ligands. Proc Natl Acad Sci USA 2002, 99: 10042-10047. 10.1073/pnas.142005099PubMed CentralView ArticlePubMedGoogle Scholar
- Sodetz JM, Pizzo SV, McKee PA: Relationship of sialic acid to function and in vivo survival of human factor VIII/von Willebrand factor protein. J Biol Chem 1977, 252: 5538-5546.PubMedGoogle Scholar
- Nossent AY, van Marion V, van Tilburg NH, Rosendaal FR, Bertina RM, van Mourik JA, Eikenboom HC: von Willebrand factor and its propeptide: the influence of secretion and clearance on protein levels and the risk of venous thrombosis. J Thromb Haemost 2006, 4: 2256-2262. 10.1111/j.1538-7836.2006.02273.xView ArticleGoogle Scholar
- Vlot AJ, Mauser-Bunschoten EP, Zarkova AG, Haan E, Kruitwagen CL, Sixma JJ, van den Berg HM: The half-life of infused factor VIII is shorter in hemophiliac patients with blood group O than in those with blood group A. Thromb Haemost 2000, 83: 65-69.PubMedGoogle Scholar
- O'Donnell JS, McKinnon TA, Crawley JT, Lane DA, Laffan MA: Bombay phenotype is associated with reduced plasma-VWF levels and an increased susceptibility to ADAMTS13 proteolysis. Blood 2005, 106: 1988-1991. 10.1182/blood-2005-02-0792View ArticlePubMedGoogle Scholar
- Bowen DJ: An influence of ABO blood group on the rate of proteolysis of von Willebrand factor by ADAMTS13. J Thromb Haemost 2003, 1: 33-40. 10.1046/j.1538-7836.2003.00007.xView ArticlePubMedGoogle Scholar
- Federici AB, Elder JH, De Marco L, Ruggeri ZM, Zimmerman TS: Carbohydrate moiety of von Willebrand factor is not necessary for maintaining multimeric structure and ristocetin cofactor activity but protects from proteolytic degradation. J Clin Invest 1984, 74: 2049-2055.PubMed CentralView ArticlePubMedGoogle Scholar
- Horwich L, Evans D, McConnell R, Donohoe W: ABO blood groups in gastric bleeding. Gut 1966, 7: 680-685. 10.1136/gut.7.6.680PubMed CentralView ArticlePubMedGoogle Scholar
- Evans D, Horwich L, McConnell R, Bullen M: Influence of the ABO blood groups and secretor status on bleeding and on perforation of duodenal ulcer. Gut 1968, 9: 319-322. 10.1136/gut.9.3.319PubMed CentralView ArticlePubMedGoogle Scholar
- Ionescu DA, Ghitescu M, Marcu I, Xenakis A: Erythrocyte rheology in acute cerebral thrombosis. Effects of ABO blood groups. Blut 1979, 39: 351-357. 10.1007/BF01014198View ArticlePubMedGoogle Scholar
- Meade TW, Cooper JA, Stirling Y, Howarth DJ, Ruddock V, Miller GJ: Factor VIII, ABO blood group and the incidence of ischaemic heart disease. Br J Haematol 1994, 88: 601-607.View ArticlePubMedGoogle Scholar
- Whincup PH, Cook DG, Phillips AN, Shaper AG: ABO blood group and ischaemic heart disease in British men. BMJ 1990, 300: 1679-1682.PubMed CentralView ArticlePubMedGoogle Scholar
- Garrison RJ, Havlik RJ, Harris RB, Feinleib M, Kannel WB, Padgett SJ: ABO blood group and cardiovacular disease: the Framingham study. Atheroslerosis 1976, 25: 311-318. 10.1016/0021-9150(76)90036-8View ArticleGoogle Scholar
- Jager A, van Hinsbergh VW, Kostense PJ, Emeis JJ, Yudkin JS, Nijpels G, Dekker JM, Heine RJ, Bouter LM, Stehouwer CD: von Willebrand factor, C-reactive protein, and 5-year mortality in diabetic and nondiabetic subjects: the Hoorn Study. Arterioscler Thromb Vasc Biol 1999, 19: 3071-3078.View ArticlePubMedGoogle Scholar
- Jick H, Slone D, Westerholm B, Inman WH, Vessey MP, Shapiro S, Lewis GP, Worcester J: Venous thromboembolic disease and ABO blood type. A cooperative study. Lancet 1969, 1: 539-542. 10.1016/S0140-6736(69)91955-2View ArticlePubMedGoogle Scholar
- Wautrecht JC, Galle C, Motte S, Dereume JP, Dramaix M: The role of ABO blood groups in the incidence of deep vein thrombosis. Thromb Haemost 1998, 79: 668-669.Google Scholar
- Larsen TB, Johnsen SP, Gislum M, Moller CA, Larsen H, Sorensen HT: ABO blood groups and risk of venous thromboembolism during pregnancy and the puerperium. A population-based, nested case-control study. J Thromb Haemost 2005, 3: 300-304. 10.1111/j.1538-7836.2005.01195.xView ArticlePubMedGoogle Scholar
- Schleef M, Strobel E, Dick A, Frank J, Schramm W, Spannagl M: Relationship between ABO and Secretor genotype with plasma levels of factor VIII and von Willebrand factor in thrombosis patients and control individuals. Br J Haematol 2004, 128: 100-107. 10.1111/j.1365-2141.2004.05249.xView ArticleGoogle Scholar
- Morelli VM, De Visser MC, Vos HL, Bertina RM, Rosendaal FR: ABO blood group genotypes and the risk of venous thrombosis: effect of factor V Leiden. Thromb Haemost 2005, 3: 183-185. 10.1111/j.1538-7836.2004.01071.xView ArticleGoogle Scholar
- Koster T, Blann AD, Briet E, Vandenbroucke JP, Rosendaal FR: Role of clotting factor VIII in effect of von Willebrand factor on occurrence of deep-vein thrombosis. Lancet 1995, 345: 152-155. 10.1016/S0140-6736(95)90166-3View ArticlePubMedGoogle Scholar
- Folsom AR, Wu KK, Rosamond WD, Sharrett AR, Chambless LE: Prospective study of hemostatic factors and incidence of coronary heart disease: the Atherosclerosis Risk in Communities (ARIC) Study. Circulation 1997, 96: 1102-1108.View ArticlePubMedGoogle Scholar
- Vischer UM: Von Willebrand factor, endothelial dysfunction, and cardiovascular disease. J Thromb Haemost 2006, 4: 1186-1193. 10.1111/j.1538-7836.2006.01949.xView ArticlePubMedGoogle Scholar
- Tirado I, Mateo J, Soria JM, Oliver A, Martinez-Sanchez E, Vallve C, Borrell M, Urrutia T, Fontcuberta J: The ABO blood group genotype and factor VIII levels as independent risk factors for venous thromboembolism. Thromb Haemost 2005, 93: 468-474.PubMedGoogle Scholar
- Ohira T, Cushman M, Tsai MY, Zhang Y, Heckbert SR, Zakai NA, Rosamond WD, Folsom AR: ABO blood group, other risk factors and incidence of venous thromboembolism: the Longitudinal Investigation of Thromboembolism Etiology (LITE). J Thromb Haemost 2007, 5: 1455-1461. 10.1111/j.1538-7836.2007.02579.xView ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.