The adaptor protein Disabled-2: new insights into platelet biology and integrin signaling

Discovery and the protein properties of Dab2

Human dab2 gene is located at the chromosome 5p13 and was first identified by Mok et al. as the tumor suppressor gene of the ovary cancer in 1994 [11]. The mouse Dab2 protein was then revealed in 1995 during the analysis of phosphoproteins induced by colony-stimulating factor-1 (CSF-1) in macrophage [12]. In 1998, Tseng et al. further defined rat dab2 as the differentially expressed gene that was up-regulated in the castrated rat prostate [13]. At least two Dab2 isoforms with the molecular weight of 82 and 59 kDa, referred to p82-Dab2 and p59-Dab2, respectively, are generated through alternative splicing (Fig. 1) [14]. Because of the undefined post-translational protein modification, the protein bands of p82-Dab2 and p59-Dab2 are up-shifted to the positions at 96 and 67 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Hence, p82-Dab2 and p59-Dab2 sometimes are referred to p96-Dab2 and p67-Dab2, respectively. The ninth coding exon corresponding to the amino acids 230–447 of p82-Dab2 is not present in the protein of p59-Dab2. As a result, several binding sites for endocytic proteins are absent in p59-Dab2. Particular motifs mediating protein-protein and protein-lipid interactions are present in Dab2, allowing them to communicate with other signaling molecules. The phosphotyrosine binding (PTB) domain is located at the N-terminus of Dab2, playing a role in the interaction of Dab2 with DIP1/2, Smad2/3, Dishevelled-3, phosphatidylinositol 4,5-bisphosphate (PI(4,5)P₂), and a subset of receptors such as integrin, low density lipoprotein receptor, megalin and related receptors that contain the non-tyrosine-phosphorylated NPXY motif [14–21]. The aspartic acid-proline-phenylalanine (DPF) motif of Dab2 interacts with the α-adaptin subunit of the clathrin adaptor protein 2 (AP-2) [21]. The C-terminal proline-rich domain (PRD) interacts with Grb2, c-Src, Akt and c-Cbl-interacting protein of 85 kDa [22–26]. By interacting with other cellular factors through these motifs, Dab2 elicits its functions in endocytosis, differentiation, and immune response and is involved in the cell signaling pathways of Ras-mitogen activated protein kinase (MAPK), Wnt, TGF-β, c-Src and RhoA-ROCK [24, 27–36]. Dab2 is also known to regulate cytoskeleton reorganization by binding to non-muscle myosin heavy chain IIA, myosin VI, actin, and dynein [12, 37–39].

Dab2 is a phosphoprotein with several phosphoryation sites having been identified. Dab2 is phosphorylated at serine residues in murine macrophage cell line in response to mitogenic stimulation by CSF-1 [12]. Dab2-Ser24 is phosphorylated by PKCβII, γ and δ but not by casein kinase II, playing a critical role in the inhibition of 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced AP-1 activity and integrin activation [33, 40]. Dab2 is hyperphosphorylated by the cyclin-dependent serine/threonine kinase Cdc2 during the mitosis phase of the cell cycle in HeLa S3 cells [41]. The phosphorylated Dab2 interacts with the peptidylprolyl isomerase Pin1 that facilitates Dab2 dephosphorylation immediately after the end of mitosis phase [42]. Similarly, Akt interacts with PRD domain of Dab2 and phosphorylates Ser448/Ser449 to regulate albumin endocytosis and mediate albumin uptake in proximal tubule [25, 43]. These distinct protein properties facilitate the involvement of Dab2 in diverse signaling network in response to extracellular responses.

Expression pattern of Dab2 in megakaryocytes and platelets

The evolutionary roles for an increase in Dab2 expression from mouse to rat and human platelets and the species-specific expression of Dab2 isoforms are not yet understood. Distinctive functions of p82-Dab2 and p59-Dab2 have been unveiled in several studies. p82-Dab2 is known to regulate receptor-mediated endocytosis, while p59-Dab2 is a transcriptional regulator when the F9 cells are undergone differentiation [18, 20, 45, 46]. Knock-in expression of p59-Dab2 only partially compromises the absence of Dab2 in the Dab2-knockout mice [14]. The increased expression of p82-Dab2 protein in human platelets may fine tune platelet response to soluble agonists and provide a superior way to prevent excessive blood loss in the large mammals. Future study using an in vivo animal model expressing human platelet p82-Dab2 should provide new insight for the aforementioned hypothesis.

Dab2 functions in megakaryocytic differentiation and platelet signaling

Dab2 has been shown to elicit multiple functions in megakaryocytic differentiation and platelet signaling (Table 1 and Fig. 2). The human K562 leukemic cells induced by TPA to form megakaryocyte-like cells have been used to define the role of Dab2 in integrin activation, cell adhesion, fibrinogen uptake, and megakaryocytic differentiation [31–34]. Dab2 is crucial in cell-cell adhesion of K562 cells and negatively regulates integrin αIIbβ3 activation and cell adhesion to fibrinogen. A mutual regulation between Dab2 and MAPK was also unveiled when the K562 cells are undergone megakaryocytic differentiation [31, 32]. Dab2 colocalizes with clathrin and mediates fibrinogen uptake in the primary megakaryocytes and megakaryocytic differentiating K562 cells [34]. Dab2-associated regulatory circuit controls mesoderm and megakaryocytic differentiation by regulating β-catenin and plakoglobin cellular distribution through the interaction between the PTB domain of Dab2 and the Asn-Pro-Asp-Tyr motif of plakoglobin [35]. Accordingly, down-regulation of Dab2 in murine ESCs disrupts cell-cell adhesion and affects embryoid body and colony formation leading to impaired mesodermal and megakaryocytic differentiation. Multiple roles of Dab2 in intracellular signaling, integrin activation and fibrinogen uptake of the megakaryocytic cells, and the fate determination of mesodermal and megakaryocytic differentiation were defined by these studies.

Dab2 is present in the cytoplasm and α-granules of human platelets and is released from the platelets in response to platelet activation [10]. Dab2 is shown in a number of studies playing a pivotal role in the activation of human platelets. Dab2 interacts with the cytoplasmic tail of the platelet integrin and regulates inside-out signaling [33]. On the other hand, Dab2 released from the α-granules is able to bind the extracellular region of αIIbβ3 integrin through the Dab2 cell-adhesion Arg-Gly-Asp (RGD) motif (amino acid residues 64–66) and the fibrinogen binding region of integrin αIIb. Such interactions compete for the integrin αIIb-fibrinogen interactions and inhibit platelet aggregation induced by soluble agonists except thrombin. Thrombin renders Dab2 inactive by cleavage of Dab2 at the amino acid residue 64 [10]. Notably, the action of thrombin on Dab2 cleavage is suppressed when Dab2 binds to the phospholipid sulfatide through four positively charged residues (Lys25, Lys49, Lys51, and Lys53) located at the PTB domain [47]. Hence, two pools of Dab2 is present at the outer surface of the platelet plasma membrane, one at the sulfatide-bound and the other at the integrin αIIbβ3-bound states. The balance between these two Dab2 states is involved in the regulation of clotting reaction, platelet aggregation and the interactions of platelet and fibrinogen in response to stimulation by platelet agonists [10, 47–49].

Megakaryocyte/platelet lineage-restricted Dab2 knockout (Dab2^-/-) mice have been generated by using the Cre-loxP transgenic system driven by the platelet factor 4 promoter to elucidate Dab2 functions in vivo [9]. Dab2^-/- platelets, when stimulated by low concentrations of thrombin, are defective in platelet aggregation, clot retraction, and spreading on fibrinogen. The functional imperfection of Dab2^-/- platelets is related to the defective responses to thrombin-induced RhoA-ROCKII and Akt-mTOR activation, ADP release and integrin αIIbβ3 activation [9]. Although Dab2 elicits a restrictive function to the murine platelets stimulated by low concentrations of thrombin, defective Dab2 expression has profound effects on hemostasis and thrombosis in vivo. This is evidenced by the observations that bleeding time is prolonged and thrombus formation is impaired in the Dab2^-/- mice. These findings are consistent with the perception that protease-activated receptors (PAR) 3- and PAR4-deficient mice, despite eliciting a partial decrease in thrombin-induced platelet aggregation, are impaired in hemostasis and are protected against thrombosis [50, 51]. These studies support the notion that Dab2 is a key regulator in hemostasis and thrombosis.

The consensuses, controversies and future prospects

The study linking Dab2 functions with megakaryocytic differentiation, platelet signaling and integrin activation was first reported 15 years ago [31]. The studies up-to-date support extensively that Dab2 is a regulator of megakaryocytic differentiation and platelet function. Nevertheless, distinctive functions of Dab2 in human and mouse megakaryocytes and platelets are noted. Knockdown of Dab2 results in an increase in αIIbβ3 activation and cell adhesion to fibrinogen in the K562 cells [33]. Dab2 is, however, required for murine platelet aggregation and integrin αIIbβ3 activation when the platelets are stimulated by low concentrations of thrombin [9]. Dab2 negatively regulates human platelet-fibrinogen interaction and platelet aggregation induced by soluble agonists except thrombin [10, 47–49]. The complexity in the abundance and species-specific expression of Dab2 isoforms and the diverse thrombin signaling in human and murine platelets [6, 9] likely contribute to the reported heterogeneity of Dab2 function. Alternatively, the discrepancy in these findings could be due to the different assay systems being used in these studies. In the studies of human platelets, recombinant Dab2 protein was the main tool for analyzing the effects of Dab2 function on platelet response by in vitro experiments [10, 47–49]. There are no Dab2 mutants or Dab2-deficient human platelets available for in vivo study. On the other hand, Dab2-deficient murine platelets have been generated for analysis of Dab2 function in vivo [9]. An animal model expressing human platelet Dab2 should help us to understand the distinctive Dab2 functions in human and murine platelets.

Dab2 is known as a phosphoprotein in a variety of cellular processes [12, 25, 33, 40–43]. Due to the lack of appropriate tools, the physiological functions of Dab2 phosphorylation in platelet signaling have not yet been elucidated. We have addressed these issues in our recent work and revealed that Dab2 is phosphorylated during agonist-stimulated human platelet activation (unpublished data). With the new tools such as CRISPR and TALEN [52–55] in the generation of genetically modified animals, the progress in gaining new insights into the signaling network involving Dab2 expression and phosphorylation in megakaryocyte, platelet biology and integrin signaling is expected to accelerate.